Issue 18, 2002
From the journal:

Chemical Communications

An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction†

Ryan G. Kruger,a   Patrick Dostala  and  Dewey G. McCafferty*a  

* Corresponding authors

a Johnson Research Foundation and the Department of Biochemistry and Biophysics, The University of Pennsylvania School of Medicine, Philadelphia, PA, USA
E-mail: deweym@mail.med.upenn.edu
Fax: +1 215-573-8052
Tel: +1 215-898-7619

Abstract

An economical and preparative-scale orthogonal solid-phase method of incorporating carboxyrhodamine and carboxyfluorescein fluorescence resonance energy transfer (FRET) probes site-specifically into synthetic peptide substrates for the S. aureus Sortase transpeptidase SrtA has been developed.

Graphical abstract: An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction

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Article information

DOI
https://doi.org/10.1039/B206303D
Article type
Communication
Submitted
01 Jul 2002
Accepted
30 Jul 2002
First published
19 Aug 2002

Chem. Commun., 2002, 2092-2093

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An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction

R. G. Kruger, P. Dostal and D. G. McCafferty, Chem. Commun., 2002, 2092 DOI: 10.1039/B206303D

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