Jump to main content
Jump to site search

Issue 1, 2002
Previous Article Next Article

Solid-phase microextraction as a sample preparation strategy for the analysis of seleno amino acids by gas chromatography-inductively coupled plasma mass spectrometry

Author affiliations

Abstract

Solid-phase microextraction (SPME) is used as a sample preparation strategy for gas chromatographic (GC) analysis of the seleno amino acids, selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys). Acylation of the amino group and esterification of the carboxylic group in these compounds was performed with isobutylchloroformate to increase volatility. The amino acid derivatives were then extracted by silica fibers with polydimethylsiloxane (PDMS) coatings prepared by the sol–gel process. Investigations of extraction time, acid and salt addition, and polymer length (for the sol–gel process) were conducted with the goal of procedural optimization. Initial characterizations were conducted using gas chromatography with flame ionization detection (GC-FID). Inductively coupled plasma mass spectrometric detection was employed for final selenium detection. Sub-ppb detection limits were obtained for all analytes although relative standard deviations were higher than those typically obtained in solid-phase microextraction.

Back to tab navigation

Publication details

The article was received on 28 Aug 2001, accepted on 08 Nov 2001 and first published on 10 Dec 2001


Article type: Paper
DOI: 10.1039/B107781C
Analyst, 2002,127, 49-53

  •   Request permissions

    Solid-phase microextraction as a sample preparation strategy for the analysis of seleno amino acids by gas chromatography-inductively coupled plasma mass spectrometry

    A. P. Vonderheide, M. Montes-Bayon and J. A. Caruso, Analyst, 2002, 127, 49
    DOI: 10.1039/B107781C

Search articles by author

Spotlight

Advertisements