Chemiluminescence biosensor chip based on a microreactor using carrier air flow for determination of uric acid in human serum

Abstract

A chemiluminescence biosensor on a chip coupled to a microfluidic system and a microreactor is described in this paper. The chemiluminescence biosensor measured 25 × 75 × 6.5 mm in dimension, and was readily produced in an analytical laboratory. The sol–gel method is introduced to co-immobilize horseradish peroxidase (HRP) and luminol in the microreactor, and to immobilize uricase in the enzymatic reactor. The main characteristic of the biosensor was to introduce air as the carrier flow instead of the more common solution carrier for the first time. The uric acid was determined by a chemiluminscent (CL) reaction between the hydrogen peroxide produced from the enzymatic reactor and luminol under the catalysis of HRP in the microreactor. The linear range of the uric acid concentration was 1 to 100 mg L⁻¹ and the detection limit was 0.1 mg L⁻¹ (3σ).