Issue 9, 2002

Chemiluminescence biosensor chip based on a microreactor using carrier air flow for determination of uric acid in human serum

Abstract

A chemiluminescence biosensor on a chip coupled to a microfluidic system and a microreactor is described in this paper. The chemiluminescence biosensor measured 25 × 75 × 6.5 mm in dimension, and was readily produced in an analytical laboratory. The sol–gel method is introduced to co-immobilize horseradish peroxidase (HRP) and luminol in the microreactor, and to immobilize uricase in the enzymatic reactor. The main characteristic of the biosensor was to introduce air as the carrier flow instead of the more common solution carrier for the first time. The uric acid was determined by a chemiluminscent (CL) reaction between the hydrogen peroxide produced from the enzymatic reactor and luminol under the catalysis of HRP in the microreactor. The linear range of the uric acid concentration was 1 to 100 mg L−1 and the detection limit was 0.1 mg L−1 (3σ).

Article information

Article type
Paper
Submitted
09 Apr 2002
Accepted
01 Jul 2002
First published
02 Aug 2002

Analyst, 2002,127, 1176-1179

Chemiluminescence biosensor chip based on a microreactor using carrier air flow for determination of uric acid in human serum

Y. Lv, Z. Zhang and F. Chen, Analyst, 2002, 127, 1176 DOI: 10.1039/B203501B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements