Issue 10, 2002
From the journal:

Analyst

Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Waraporn Putalun,a   Hiroyuki Tanaka,b   Toshiya Muranakac  and  Yukihiro Shoyama*b  

* Corresponding authors

a Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

b Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan
E-mail: shoyama@phar.kyushu-u.ac.jp
Fax: +81-92-642-6580
Tel: +81-92-642-6580

c Plant Science Center, RIKEN (The Institute of Physical and Chemical Research) 2-1, Hirosawa, Wako, Saitama, Japan

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for determination of aculeatisides. Aculeatiside A was conjugated with bovine serum albumin (BSA) for immunization. The ratio of hapten in an antigen conjugate was determined by matrix-assisted laser desorption/ionization TOF mass spectrometry. Polyclonal antibody was developed in rabbits against an aculeatiside A–BSA conjugate. The antibody was specific for aculeatiside A and aculeatiside B. The range of the immunoassay extended from 100 ng ml−1 to 5 µg ml−1 of aculeatisides. Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples.

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Article information

DOI
https://doi.org/10.1039/B203351H
Article type
Paper
Submitted
04 Apr 2002
Accepted
25 Jul 2002
First published
30 Aug 2002

Analyst, 2002,127, 1328-1332

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Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

W. Putalun, H. Tanaka, T. Muranaka and Y. Shoyama, Analyst, 2002, 127, 1328 DOI: 10.1039/B203351H

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