Issue 7, 2002

Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Abstract

We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

Article information

Article type
Paper
Submitted
25 Mar 2002
Accepted
09 May 2002
First published
27 May 2002

Analyst, 2002,127, 972-976

Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

K. Nakajima, Y. Oda, M. Kinoshita, T. Masuko and K. Kakehi, Analyst, 2002, 127, 972 DOI: 10.1039/B202950B

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