Metabolic profiling of rodent biological fluids via1H NMR spectroscopy using a 1 mm microlitre probe

Abstract

The application of a 1 mm TXI (¹H/¹³C/¹⁵N) microlitre NMR probe with z-gradient for metabolic profiling of biofluids is described. The probe was used to provide spectral profiles for rat blood plasma using only ∼2 μl of fluid with a range of solvent suppression techniques. Using a similar amount of fluid, spectra were obtained from rat and mouse cerebrospinal fluid, demonstrating that the probe could be used to profile rodents metabolically via biofluids previously inaccessible to NMR analysis without the need for termination.