Preparation and spectroscopic studies of an inclusion complex of adenine with β-cyclodextrin in solution and in the solid state

Abstract

The interaction between adenine and β-CD has been investigated in solution and in the solid state by several analytical techniques, primarily by ¹H-NMR, 2D ROESY and fluorescence spectra, and secondarily by other important techniques, for example, Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). The association constant and 1 ∶ 1 nature of the complex between adenine and β-CD in solution were determined by fluorescence spectroscopy. A spatial configuration for the complex in solution is proposed from analysis of the ¹H-NMR and 2D ROESY data. The Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) data are consistent with the formation of an inclusion complex. In addition, a solid inclusion complex of adenine with β-CD was synthesized by the coprecipitation method.