ESMS and NMR investigations on the interaction of the anticancer drug cisplatin and chemopreventive agent selenomethionine

Abstract

The reactions of L-selenomethionine (L-Se-MetH) with cisplatin, cis-[PtCl₂(NH₃)₂], were investigated using electrospray mass spectroscopy (ESMS) and 2-D [¹H–¹⁵N] NMR spectroscopy. The reaction intermediates and products identified were cis-[PtCl(L-Se-MetH)(NH₃)₂]⁺, cis-[Pt(L-Se-Met)(NH₃)₂]⁺, [PtCl(L-Se-MetH)(NH₃)]⁺ and [Pt(L-Se-Met)(L-Se-MetH)]⁺. Some binuclear adducts were also detected by ESMS during the reaction, although they existed in minor amounts. Similar reactions with L-MetH were also conducted under similar conditions for comparison. This work provides the first detailed studies on the reaction of a platinum-based drug with L-Se-MetH.