The controlled glycosylation of a protein with a bivalent glycan: towards a new class of glycoconjugates, glycodendriproteinsSome of this work was presented at a joint meeting of the RSC Carbohydrate and Bioorganic groups, Warwick, July 6, 2000 and at the 20th International Carbohydrate Symposium, Hamburg, August 30, 2000.Electronic supplementary information (ESI) available: selected NMR data. See http://www.rsc.org/suppdata/cc/b0/b009184g/

Abstract

The use of a novel bivalent carbohydrate modification reagent, based on a flexible, branched divalent core in a combined site-directed mutagenesis and chemical modification strategy has allowed the first controlled synthesis of a pure protein bearing a branched glycan or a first generation glycodendriprotein.