Incorporation of [U-13C]glycerol defines plausible early steps for the biosynthesis of methylenomycin A in Streptomyces coelicolor A3(2)Electronic supplementary information (ESI) available: HMBC, 1H and 13C NMR spectra of methylenomycin A. See http://www.rsc.org/suppdata/cc/b1/b101615f/

Abstract

Incorporation of [U-¹³C]glycerol into the linear plasmid-encoded secondary metabolite methylenomycin A, both intact and indirectly via metabolism to acetyl CoA, was observed in Streptomyces coelicolor suggesting plausible early biosynthetic steps for this unusual antibiotic.