High performance liquid chromatographic determination of mazindol in human plasma

Abstract

A simple and convenient high performance liquid chromatographic method with UV detection is described for the determination of mazindol {5-(p-chlorophenyl)-2,5-dihydro-3H-imidazo[2,1-a]isoindol-5-ol} and its major metabolite, 2-(2-aminoethyl)-3-(p-chlorophenyl)-3-hydroxyphthalimidine (Met), in human plasma. The analytes were extracted with ethyl acetate from plasma samples and separated on a C₁₈ column using acetonitrile–0.067 mol dm⁻³ phosphate buffer (pH 3.5) (24 + 76 v/v) as a mobile phase. The eluates were monitored at 220 nm. Following complete validation and stability studies, the proposed method proved to be sensitive and precise. The limits of detection were 0.07 and 0.08 ng ml⁻¹ of plasma for mazindol and Met, respectively. The accuracy and recovery were in the ranges 94–102% and 91–102%, respectively, for both compounds. The intra- and inter-assay precisions were less than 7.6 and 9.2%, respectively, for both compounds. The stability of mazindol under different storage conditions, i.e., at room temperature (rt) and 4 °C and with freeze–thaw cycles, was also examined. Mazindol was unstable in plasma samples left at rt and 4 °C. The method was applied to the determination of mazindol and Met in the plasma of a patient treated for obesity with mazindol.