Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 9, 2001
Previous Article Next Article

Separation and determination of protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza by capillary electrophoresis with amperometric detection

Author affiliations

Abstract

Capillary electrophoresis with amperometric detection was applied to separate and determine protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza preparations. The electrode used was a 0.3 mm diameter carbon disk electrode fixed in a wall-jet with amperometric detection. Under the optimum conditions, the two analytes were separated completely within 8 min. Excellent linearity was obtained in the concentration ranges of 0.25–100.0 µg ml−1 and 0.50–100.0 µg ml−1 for protocatechuic aldehyde and protocatechuic acid, respectively. The detection limits were 0.10 µg ml−1 of protocatechuic aldehyde and 0.25 µg ml−1 of protocatechuic acid, which were found to be lower than those of other methods that determine protocatechuic aldehyde (3,4-dihydroxybenzaldehyde) and protocatechuic acid (3,4-dihydrobenzoic acid) simultaneously. The mean recoveries of protocatechuic aldehyde and protocatechuic acid were 97.4% and 103.3%. This method has been successfully applied to monitor these two components in real samples such as Salivia miltorrhrza and its two traditional Chinese medicinal preparations.

Back to tab navigation

Article information


Submitted
05 Dec 2000
Accepted
08 Jun 2001
First published
25 Jul 2001

Analyst, 2001,126, 1519-1523
Article type
Paper

Separation and determination of protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza by capillary electrophoresis with amperometric detection

Y. Pan, L. Zhang and G. Chen, Analyst, 2001, 126, 1519
DOI: 10.1039/B009760H

Social activity

Search articles by author

Spotlight

Advertisements