Issue 4, 2001

Characteristics of a column suitable for capacity gradient chromatography with a borate eluent

Abstract

In capacity gradient elution, the gradient separation of ionic species is achieved by decreasing the ion-exchange capacity of a column during the course of the separation. Diol-type hydroxy groups on the resin surface form anionic complexes with borate as an eluting reagent. Thus, a chemically bonded anion-exchange column enriched with residual hydroxy groups allows the creation of a capacity gradient. An increase in the amount of the complex formed gradually brings about a decrease in the ion-exchange capacity of the column, and strongly bound analyte ions are eluted. We investigated the characteristics of a column suitable for this eluent system. The concentration of borate eluent required to remove the ion-exchange capacity depended inversely on the ratio of the residual hydroxy groups to functional groups. On a column in which this ratio was approximately 100, the ion-exchange capacity could easily be adjusted by using a low concentration of mannitol as a competing reagent. Use of this column led to very small baseline shifts during the borate–mannitol gradients, and to the simultaneous determination of anions with widely varying retention times.

Article information

Article type
Paper
Submitted
20 Oct 2000
Accepted
15 Feb 2001
First published
12 Mar 2001

Analyst, 2001,126, 465-468

Characteristics of a column suitable for capacity gradient chromatography with a borate eluent

A. Yamamoto, S. Kodama, A. Matsunaga, Y. Inoue, T. Aoyama and Y. Kumagai, Analyst, 2001, 126, 465 DOI: 10.1039/B008478F

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