Study of the separation and determination of monosaccharides in soluble coffee by capillary zone electrophoresis with electrochemical detection

Abstract

A simple, fast and reliable method, based on capillary electrophoresis with electrochemical detection, for the separation and determination of six monosaccharides, namely glucose, galactose, arabinose, fructose, xylose and ribose, in soluble coffees was developed. A copper disk electrode was used as the working electrode. The optimum conditions for separation and detection were 50 mmol L⁻¹ sodium hydroxide buffer (pH 12.7), separation voltage 5 kV and detection potential 0.65 V (vs. Ag/AgCl). The linear ranges were from 5.0 × 10⁻³ to 0.5 mmol L⁻¹ for all six sugars. All regression coefficients were >0.99. The detection limits for all the sugars were 1.0 × 10⁻³ mmol L⁻¹. The RSD of the peak current was <4.2% (n = 5). The proposed method was applied directly to the separation and determination of the six sugars without prior derivatization, and the assay results were satisfactory.