The enhanced electrogenerated chemiluminescence of Ru(bpy)32+ by glutathione on a glassy carbon electrode modified with some porphine compounds

Abstract

It has been found that the electrochemical activity of glutathione was increased greatly at the glassy carbon electrodes modified with 5,10,15,20-tetraphenylporphine ruthenium(II) carbenyl (RuTPP), meso-tetraphenylporphine copper(II) complex (CuTTP) and hemin. It has been also found that glutathione would enhance the electrogenerated chemiluminescence (ECL) of Ru(bpy)₃²⁺ at a hemin glassy carbon electrode; the enhanced ECL intensity was linear with the concentration of glutathione in the range of 1 × 10⁻⁷ ∼ 1 × 10⁻⁴ mol l⁻¹, based on which method for determination of glutathione has been developed. The detection limit of glutathione was 2 × 10⁻⁸ mol l⁻¹, and the relative standard deviation for 1 × 10⁻⁶ mol l⁻¹ glutathione was 2.7%. The mechanism for this ECL system has been proposed.