Study of the fluorescence characteristics of norfloxacin in reversed micelles and application in analysis

Abstract

The fluorescence characteristics of norfloxacin (NFLX) were studied in reversed micelles which formed with sodium bis(2-ethylhexyl) sulfosuccinate (AOT)–water–octane. The influences of the environmental factors, such as the water content, AOT concentration and pH, on the fluorescence of NFLX were investigated. A novel spectrofluorimetric method for the direct determination of NFLX is proposed based on the enhancement effect of reversed micelles on the native fluorescence of NFLX, which showed a much lower detection limit (0.0032 μg mL⁻¹) and a wider linear range (0.015–3.8 μg mL⁻¹) compared with aqueous solution.