Measurement of nitric oxide released in the rat heart with an amperometric microsensor

Abstract

Nitric oxide (NO) plays an important role in various physiological processes, acting either as an intra- and intercellular messenger or as a toxic agent. The detection and quantification of NO have been accomplished by a variety of methodologies. In the present study, real-time production of NO in the rat heart was continuously measured by using a novel copper–platinum microparticle-modified NO electrochemical microsensor. The linearity range of the microsensor is between 8.0×10⁻⁸ and 4.8×10⁻⁶ mol L⁻¹ and the detection limit is 3.0 × 10⁻⁸ mol L⁻¹. NO release from the rat heart stimulated by the agonists L-arginine and acetylcholine was observed, and the responses were decreased by the NO synthase inhibitor L-N^ω-nitroarginine. In addition, the effect of sodium nitroprusside (SNP), a NO donor, was also studied. SNP increases the concentration of NO in the rat heart. The experiments showed that electrochemical detection is suitable for detecting and quantifying NO in biological systems.