New chemoenzymatic approach to glyco-lipopolymers: practical preparation of functionally active galactose–poly(ethylene glycol)–distearoylphosphatidic acid (Gal–PEG–DSPA) conjugate
Abstract
A practical approach to galactose–PEG–distearoylphosphatidic acid (DSPA) retaining full lectin binding, involves glycosylation of monobenzyl ether–PEG, suitable protection of the sugar hydroxy groups, debenzylation, followed by enzymatic transphosphatidylation with phosphatidylcholine and final deprotection.