Sphingosine 1-phosphate was prepared from sphingosine by two steps involving conversion of sphingosine to phosphatidylsphingosine catalyzed by phospholipase D and enzymatic hydrolysis of the intermediate to a diacyglycerol and sphingosine 1-phosphate with phospholipase C.
E. Morigaki, Y. Miura, K. Takahata, M. Tada, S. Nakajima and N. Baba, J. Chem. Res. (S), 1998, 774 DOI: 10.1039/A804770G
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