Jump to main content
Jump to site search

Issue 12, 1998
Previous Article Next Article

In vitro liver models are important tools to monitor the abuse of anabolic steroids in cattle†

Abstract

Current veterinary residue analysis mainly focuses on the monitoring of residues of the administered parent compound. However, it is possible that larger amounts of metabolites are excreted and that they can have a prolonged excretion period. In order to unravel specific metabolic steps and to identify possible biological markers, two in vitro liver models were used, i.e. monolayer cultures of isolated hepatocytes and liver microsomes, both prepared from liver tissue of cattle. Clostebol, boldenone, norethandrolone (NE) and ethylestrenol (EES) were used as model substrates. Results show that the metabolic profiles derived from in vitro experiments are predictive for the in vivo metabolic pathways of the steroids evaluated in this study. By means of this strategy, it is possible to identify 17α-ethyl-5β-estrane-3α,17β-diol (EED) as a common biological marker for NE and EES. By in vivo experiments it was shown that EED is particularly important for the detection of the abuse of NE or EES because of its high excretion levels and its prolonged presence as compared with the parent compounds or any other metabolite.

Back to tab navigation

Article type: Paper
DOI: 10.1039/A805013I
Analyst, 1998,123, 2453-2456

  •   Request permissions

    In vitro liver models are important tools to monitor the abuse of anabolic steroids in cattle†

    M. Van Puymbroeck, L. Leyssens, J. Raus, M. Van Puymbroeck, D. Vanderzande, J. Gelan, J. Raus, M. E. M. Kuilman, R. F. M. Maas, R. F. Witkamp and R. F. Witkamp, Analyst, 1998, 123, 2453
    DOI: 10.1039/A805013I

Search articles by author

Spotlight

Advertisements