Phospholipase-A2-mediated stereoselective synthesis of (R)-1-O-alkylglycero-3-phosphate and alkyl-acyl analogues: application for synthesis of radiolabelled biosynthetic precursors of cell surface glycoconjugates of Leishmania donovani

Abstract

Stereoselective syntheses of (R)-1-O-alkylglycero-3-phosphate and alkyl-acyl analogues have been achieved using glycerol as starting material by an efficient application of Phospholipase-A₂ enzyme from Naja mocambique mocambique. This synthetic strategy allows a high yielding preparation of radiolabelled [¹⁴C] and chirally pure biosynthetic precursors of GIPLs and LPG cell surface molecules of promastigote and amastigote forms of the protozoan parasite Leishmania donovani.