Oxidative damage on RNA mediated by cationic metalloporphyrin–antisense oligonucleotides conjugates†

Abstract

A manganese cationic porphyrin covalently linked to the 5′ end of an antisense oligonucleotide was shown to mediate sequence-specific oxidative lesions on a mRNA target when activated by KHSO₅. This manganese porphyrin cleaver did not induce direct RNA strand breaks but promoted the arrest of reverse transcription due to oxidative damage on bases of the RNA template.