Dissecting the effects of cooperativity on the stabilisation of a de novo designed three stranded anti-parallel β-sheet
Abstract
Cooperative effects between two sets of weak interactions at different hydrogen bonded interfaces are shown to stabilise a de novo designed three-stranded anti-parallel β-sheet: NMR and CD (circular dichroism) spectroscopy have been used to compare and contrast the stability of the β-sheet (residues 1–24) and the isolated C-terminal β-hairpin (residues 9–24).