Unique binding site for bis-benzimidazoles on transfer RNA

Abstract

Ligands based on bis-benzimidazole structures related to Hoechst 33258 bind to transfer RNA with 1:1 stoichiometry and dissociation constants in the micromolar range, their interaction appearing significantly different in nature from that with duplex DNA, based on the ribonucleic acid-induced quenching of their fluorescence.