Glycosidase-catalysed synthesis of oligosaccharides: a one step synthesis of lactosamine and of the linear B type 2 trisaccharide α-d-Gal-(1→3)-β-d-Gal-(1→4)-β-d-GlcNAcSEt involved in the hyperacute rejection response in xenotransplantation from pigs to man and as the specific receptor for toxin A from Clostridium difficile

Abstract

Sequential use of the β-galactosidase from Bacillus circulans and an α-galactosidase from Aspergillus oryzae give the linear B trisaccharide 2, identified as the epitope that binds anti-Gal antibodies during pig-to-man xenotransplantation and as the receptor for toxin A from Clostridium difficile.