Derivatization of Amphetamine and Methamphetamine With 1,2-Naphthoquinone 4-Sulfonic Acid Into Solid-phase Extraction Cartridges. Determination of Amphetamine in Pharmaceutical and Urine Samples

Abstract

The derivatization of amphetamine and methamphetamine with 1,2-naphthoquinone-4-sulfonate (NQS) into solid-phase extraction cartridges (C ₁₈ ) is described. Optimum conditions were the use of carbonate–hydrogencarbonate buffer of pH 10, a 10 min reaction time at 25 °C and an NQS concentration of 9.6 × 10 ^{- 3} M. The accuracy and the precision of the method were tested. The results obtained with the proposed liquid–solid procedure were compared with those obtained with a traditional liquid–liquid extraction with hexane–ethyl acetate. The procedure was used to measure amphetamine in pharmaceutical and urine samples