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Issue 8, 1997
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Miniaturized Graphite Sensors Doped With Metal–Bathophenanthroline Complexes for the Selective Potentiometric Determination of Uric Acid in Biological Fluids

Abstract

Miniaturized poly(vinyl chloride) matrix membrane sensors in an all-solid-state graphite support, responsive to urate anion, were developed. The membranes incorporate lipophilic ion-pair complexes of urate anion with ruthenium(III), iron(II), nickel(II) and copper(I) bathophenanthroline (4,7-diphenyl-1,10-phenanthroline) counter cations. The sensors demonstrate a near-Nernstian response to urate over the concentration range 1 × 10 - 2 –1 × 10 - 5 mol l - 1 and have micromolar detection limits and good selectivity properties. The response is virtually unaffected by pH changes in the range 7–10 and the response times are 5–10 s in aqueous solutions and in human serum and urine samples. A flow injection detector incorporating an iron(II) bathophenanthroline–urate graphite sensor was used for continuous monitoring of uric acid. The minimum detectable concentration was approximately 8 µg ml - 1 and the sample throughput was approximately 120 h - 1 . Direct potentiometric determination of uric acid in the static and hydrodynamic modes of operation over the range 15 µg ml - 1 –1.5 mg ml - 1 showed average recoveries of 98.7 and 97.8% with RSDs of 0.6 and 0.7%, respectively. Application of the method to the determination of uric acid in human serum and urine gave results that compared favourably with those obtained by the standard spectrophotometric method.

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Article information


Analyst, 1997,122, 815-819
Article type
Paper

Miniaturized Graphite Sensors Doped With Metal–Bathophenanthroline Complexes for the Selective Potentiometric Determination of Uric Acid in Biological Fluids

S. S. M. Hassan and N. M. H. Rizk, Analyst, 1997, 122, 815
DOI: 10.1039/A701038I

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