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Issue 2, 1997
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Spectrofluorimetric Determination of Vitamin K3 by a Solid-phase Zinc Reactor Immobilized in a Flow Injection Assembly

Abstract

The spectrofluorimetric determination of vitamin K 3 (menadione) using a flow injection (FI) assembly provided with a solid-phase reactor with immobilized zinc is described. The naphthohydroquinone was produced by means of two coupled steps in the FI system: hydrolysis of the sodium sulfite derivative of the menadione in a basic medium and reduction of the generated menadione in the zinc reactor in an acidic medium. The fluorescent product was monitored spectrofluorimetrically (λ ex 325 nm; λ em 425 nm). The calibration graph was linear over the range 0.1–18 µg ml - 1 with a reproducibility of 1.6%; the limit of detection was 0.005 µg ml - 1 and the sample throughput was 70 h - 1 . The influence of foreign compounds was studied and the procedure was applied to the determination of vitamin K 3 in three different pharmaceutical formulations.

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Article information


Analyst, 1997,122, 139-142
Article type
Paper

Spectrofluorimetric Determination of Vitamin K3 by a Solid-phase Zinc Reactor Immobilized in a Flow Injection Assembly

I. Gil Torró, J. V. García Mateo and J. Martínez Calatayud, Analyst, 1997, 122, 139
DOI: 10.1039/A606881B

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