Issue 3, 1997
From the journal:

Analyst

Enzymic Method for the Spectrophotometric Determination of Benzoic Acid in Soy Sauce and Pickles

Takashi Hamano,   Yukimasa Mitsuhashi,   Nobumi Aoki,   Masanori Semma  and  Yoshio Ito  

Abstract

A simple spectrophotometric method for the determination of benzoic acid is described. Benzoic acid is measured enzymically through its reaction with benzoate 4-hydroxylase coupled with NADPH and O 2 . The entire enzymic procedure required 20 min to complete. The method greatly simplifies the procedure for benzoic acid determination and permits the routine inspection of a number of samples with very little laboratory equipment. The method was compared with HPLC and satisfactory agreement was achieved.

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Article information

DOI
https://doi.org/10.1039/A606537F
Article type
Paper

Analyst, 1997,122, 259-262

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Enzymic Method for the Spectrophotometric Determination of Benzoic Acid in Soy Sauce and Pickles

T. Hamano, Y. Mitsuhashi, N. Aoki, M. Semma and Y. Ito, Analyst, 1997, 122, 259 DOI: 10.1039/A606537F

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