A convenient one-pot synthesis of cystine-containing peptides from protected peptidyl resins using the trimethylsilyl chloride–dimethyl sulfoxide–trifluoroacetic acid system

Abstract

Cystine-containing peptides are obtained in a one-pot manner by treatment of protected peptidyl resins with trimethylsilyl chloride (TMSCl)–dimethyl sulfoxide (DMSO)–trifluoroacetic acid (TFA). The TMSCl–DMSO–TFA system simultaneously cleaves peptides from the resins, with deprotection of all side chain protecting groups and disulfide bond formation.