Quantitative study of the interactions of ATP with amines and amino acids

Abstract

Interactions of some amines (ethylenediamine, putrescine, cadaverine, histamine, diethylenetriamine, spermidine, spermine, triethylenetetramine and pentaethylenehexamine) and amino acids (lysine and histidine) with ATP have been studied potentiometrically, at 25 °C. Several species A(ATP)H^(q–4)_q[A = amine or amino acid, q= 1 …n+ 2; (n= number of aminogroups)] were found. For diamine– and diethylenetriamine–ATP systems, the species A₂(ATP)H₄ are also formed. The stability of these mixed species is strictly dependent on the charges involved in the formation reaction, and empirical stability–charges relationships are reported. Effects of ionic medium on formation constants are discussed, together with the importance of interferences.