The interaction between cytochrome c and trans-[PtCl2(NH3)2]

Abstract

The reaction between cytochrome c and trans-[PtCl₂(NH₃)₂] has been studied under different conditions. The complex can not only bind to a Met-80 residue, which causes the Fe–S bond of ferricytochrome c to be broken, but can also induce a facile conversation of low-spin iron(III) cytochrome c to the high-spin form under mild conditions. Cytochrome c can also be cross-linked selectively by trans-[PtCl₂(NH₃)₂]. The binding sites of the platinum complex in the protein derivatives were identified by analysis of the molar ratio of the two metals (Pt and Fe), ¹H NMR and UV/VIS spectroscopy and differential-pulse voltammetry.