Existence of two basic sites in triazolo-1,4-diazepines: determination of two pKa values for a model compound in water
By a UV/VIS spectrophotometric study in the pH range –1.6 to 10.1 and by a polarographic study of a water soluble model compound, the occurrence of two basic sites in water has been ascertained for triazolo-1,4-diazepines. The pKa values found for this model were –0.24 and +1.81. Owing to the overlapping of the two pKa values, microforms exist simultaneously. Corresponding ionization microconstant values have been tentatively assigned.