Capillary zone electrophoresis of human recombinant erythropoietin using C8 coated columns without additives in the running buffer

Abstract

The use of C₈ coated capillary columns enabled the characterization of human recombinant erythropoietin (r-HuEPO) as well as its determination by capillary electrophoresis. Samples were injected at 90 V cm^–1 during a period at 30 s and the electrophoregrams were recorded at 210 V cm^–1. A 0.03 mol l^–1 phosphate solution at pH 7.0 (without additives) was used as the running buffer. A minimum amount of 19 ± 1 pg, which corresponds to a concentration in the sample of 0.37 µg ml^–1, was found using UV detection at 240 nm. Several experiments were carried out in order to study the behaviour of r-HuEPO in this type of capillary. r-HuEPO was determined in spiked urine samples.