Dynamic aqueous supercritical fluid extraction of the enzymic hydrolysis of testosterone-β-D-glucuronide. Analysis of liberated testosterone by gas chromatography–mass spectrometry

Abstract

The enzymic hydrolysis of testosterone-β-D-glucuronide has been performed under aqueous SFE conditions. Liberated testosterone was continuously extracted and trapped onto an ODS HPLC column. Results of quantitative GC–MS indicate that 70.1% of testosterone-β-D-glucuronide was hydrolysed after 135 min of enzymic digestion resulting in 88% of the free testosterone being trapped after 120 min of dynamic aqueous SFE.