Nitrate and nitrite determination in complex matrices by gradient ion chromatography

Abstract

Simultaneous determination of nitrate and nitrite in human serum has been accomplished using gradient ion chromatography and direct UV detection, with centrifugal ultrafiltration as the only pretreatment. The method has advantages over previous techniques in both ease of use and cost per assay. The sensitivity is adequate for use as a monitor of nitric oxide induced changes in serum nitrate and nitrite levels.