Simultaneous kinetic spectrophotometric determination of 2-furfuraldehyde and 5-hydroxymethyl-2-furfuraldehyde by application of a modified Winkler's method and partial least squares calibration

Abstract

A method is described for the simultaneous determination of 2-furfuraldehyde and 5-hydroxymethyl-2-furfuraldehyde, based on their reaction by a modified Winkler's method. A comparative study of the results found using the kinetic curves registered at 550 and 585 nm, as analytical signals, has been performed. The data set of the kinetic curves at 550 nm was selected as the analytical signal. Partial least squares (calibrating for a single chemical constituent at a time: PLS-1) multivariate calibration was then applied for the determination. The proposed method was satisfactorily applied to the analysis of wines, spirits, fruit juices and honey.