Electrogenerated chemiluminescent determination of pyruvate using tris(2,2′-bipyridine)ruthenium(II)
Abstract
A novel method for the sensitive and selective determination of pyruvate is presented, using an electrogenerated chemiluminescence (ECL) reaction of tris(2,2′-bipyridine)ruthenium(II) in the presence of cerium(III) nitrate, increasing the number and diversity of analytes currently detected by this ECL reaction. Various experimental conditions have been optimized, leading to a calibration over three-orders of magnitude of concentration, with a detection limit of 3.1 × 10–7 mol l–1(27 ppb). Simple methods have also been developed to suppress the interference effects of various compounds that are expected to be present in potential samples from fermentation media.