Perspective. Analytical validation of a general protocol for the preparation of dose-controlled solutions in aluminium toxicology

Abstract

Aluminium(III) solutions suitable for careful toxicological investigation both in vivo and in vitro, are prepared from AlCl₃·6H₂O and from neutral metal complexes, Al(lactate)₃, Al₂(citrate)₂(H₂O)₆, Al(acetylacetonate)₃ and Al(maltolate)₃, by dissolving the pure compounds in TRIS-HCl buffer solutions. The metal concentrations in the supernatant liquids are determined spectrophotometrically under reproducible conditions. Al(acetylacetonate)₃ and Al(maltolate)₃ are detected as molecular entities by using ¹H NMR spectroscopy down to concentrations of 200 and 100 µmol l^–1, respectively.