Evaluation of a split-type flow cell for a polarized spectrophotometric detector

Abstract

A split-type flow cell for a polarized spectrophotometric detector (PPD), in which the column effluent is simultaneously passed through both the sample and reference sides, is described. The improvement in detection sensitivity in PPD with the use of this cell is discussed. Its utility as a universal polarimetric detector for HPLC for the detection of coloured amino acid–copper(II) complexes is shown. A new possibility for this cell in the gradient elution separation of glucose syrup is demonstrated.