Detection and quantitative determination of catechol derivatives using an iron(III)–ethylenediamine visible absorbance assay
Abstract
A rapid and simple assay procedure based on iron(III) ion complexation for the detection and quantitative determination of catechol derivatives over the 5–50 µmol l–1 range is described. The potential interference from ascorbate, acetaminophen (paracetamol) and urate are evaluated and the role and importance of the assay components assessed.