Matrix solid phase dispersion linked to solid phase extraction for β-agonists in liver samples: an update

Abstract

An extraction procedure has been developed for β-agonists in liver samples using combined MSPD-C₁₈ SPE clean-up. The procedure is suitable for the determination of compounds from both β-agonist classes, the substituted aniline-type compounds (e.g., clenbuterol) and the phenolictype compounds (e.g., salbutamol), at levels below 1 ng g^–1. The procedure is more robust than the method recently reported from this laboratory in that it does not rely on the special properties of one particular brand of C₁₈ material. In this instance, the procedure has been evaluated using two major commercial brands of C₁₈ material. For both C₁₈ materials similar results were obtained for salbutamol, clenbuterol and mabuterol in fortified and incurred liver samples.