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Issue 13, 1994
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Cellulase-catalysed glycosylation reactions: simple route towards a highly selective synthesis of oligosaccharides

Abstract

Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non -glucosidic saccharides and modified glucoses as glycosyl acceptors also react with β-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having β(1→4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.

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Article type: Paper
DOI: 10.1039/P19940001851
Citation: J. Chem. Soc., Perkin Trans. 1, 1994,0, 1851-1857

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    Cellulase-catalysed glycosylation reactions: simple route towards a highly selective synthesis of oligosaccharides

    O. Karthaus, S. Shoda, H. Takano, K. Obata and S. Kobayashi, J. Chem. Soc., Perkin Trans. 1, 1994, 0, 1851
    DOI: 10.1039/P19940001851

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