The mechanism of Escherichia coli dethiobiotin synthetase—the closure of the ureido ring of dethiobiotin involves formation of a carbamic-phosphate mixed anhydride
Abstract
The final intermediate in the enzymatic synthesis of the ureido ring of D-dethiobiotin 1 from (7R,8S)-7,8-diaminononanoate 2 catalysed by E. coli dethiobiotin synthetase is the phosphoric acid anydride 4 of the carbamate 3.