Mechanism of dethiobiotin synthetase—characterisation of the 8-aminocarbamate of (7R,8S)-7,8 diaminononanoate as an enzyme-bound intermediate

Abstract

Enzymatically catalysed formation of the ureido ring of dethiobiotin 2 from (7R, 8S)-7,8-diaminononanoate 3 by purified E. coli dethiobiotin synthetase involves the formal hydrolysis of one equivalent of ATP, a combination of substrate loading and trapping experiments show that the carbamate 5 is an intermediate in the synthesis of dethiobiotin from 3.