An accurate method for simultaneous estimation of 31P–1H and 1H–1H vicinal coupling constants along the backbone of DNA fragments is described using the two-dimensional heteronuclear long-range correlation (2D HELCO) experiment.
K. V. R. Chary, V. K. Rastogi, G. Govil and H. T. Miles, J. Chem. Soc., Chem. Commun., 1994, 241 DOI: 10.1039/C39940000241
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