Enzyme immunoassay for the detection of levamisole in meat and milk

Abstract

Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in milk and in 100 g l^–1 muscle homogenate. The limit of detection is 1 µg l^–1. In milk the inter-assay relative standard deviation (s_r) is 6% at 10 µg kg^–1 and the intra-assay s_r value 7.2%. In tissue homogenate the inter-assay s_r value is 12.7% at 5 µg kg^–1 and the intra-assay s_r value is 3.8%. Total incubation time is 1 h.