Sensitive method for measuring lactate dehydrogenase activity in human serum by differential-pulse polarography
Abstract
A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.
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