Vapour-phase acid digestion of micro samples of biological material in a high-temperature, high-pressure asher for inductively coupled plasma atomic emission spectrometry

Abstract

This vapour-phase acid decomposition of small biological samples (50–165 mg) and concurrent purification of the reagent acid were achieved in a mini-quartz sample holder inserted in a commercial high-pressure digestion vessel. A 3.1 ml volume sample container was developed to hold the sample and to maximize the successful decomposition of a variety of biological samples. When biological standard reference materials were digested at 230 °C and 122 bar (1770 psi), the residual carbon content in the digested samples was less than 1.8 ± 0.1%. Inductively coupled plasma atomic emission spectrometric analysis of the digested materials for C, Ca, Cu, Cd, Fe, Mg, Mn and Zn provided good recoveries and low reagent blank values and demonstrated complete matrix decomposition.