Studies of the interaction of a meta-hydroxy analogue of hoechst 33258 with DNA by melting temperature, footprinting and high-resolution 1H NMR spectroscopy

Abstract

A new analogue of Hoechst 33258 1 with a small structural change, i.e., the phenolic OH group being located meta rather than para, has been shown by footprinting, DNA melting studies and high-field ¹H NMR spectroscopy to bind to the AATT sequences of DNA in a manner grossly similar to Hoechst 33258, although analysis by ¹H NMR spectroscopy indicates that the phenolic end of the ligand could be prised away from the minor groove relative to Hoechst 33258, and that the major groove structure of d(CGCGAATTCGCG)₂ is significantly perturbed by the binding of this Hoechst 33258 analogue.